A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Paracetamol (PCM) and Pamabrom (PAM) by employing first order derivative zero crossing method in 0.1 N NaOH. The first order derivative absorption at 258 nm (zero cross point of Paracetamol) was used for quantification of Pamabrom and 280 nm (zero cross point of Pamabrom) for quantification of Paracetamol. The linearity was established over the concentration range of 2-12 µg/ml and 5-30 µg/ml for Paracetamol and Pamabrom with correlation coefficient r2 0.9977 and 0.9978, respectively. The mean % recoveries were found to be in the range of 99.33 % – 101.01 % and 98.96 % – 100.67 % for Paracetamol and Pamabrom, respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of Paracetamol and Pamabrom in bulk and in synthetic mixture.
Loading....